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MAP2K3 can regulate proliferation, migration, and apoptosis of breast cancer cells. (A) <t>CCK8</t> assay shows the proliferation ability of BT474 cells after overexpressing MAP2K3. (B) Transwell assay shows the invasion ability of BT474 cells after overexpressing MAP2K3. (C) Apoptosis assay shows the apoptosis of BT474 cells after overexpressing MAP2K3. The statistical method is T ‐test. Values were expressed as the mean ± SD from three experiments, and the asterisk indicates the statistical significance compared to the controls ( ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001).
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MAP2K3 can regulate proliferation, migration, and apoptosis of breast cancer cells. (A) CCK8 assay shows the proliferation ability of BT474 cells after overexpressing MAP2K3. (B) Transwell assay shows the invasion ability of BT474 cells after overexpressing MAP2K3. (C) Apoptosis assay shows the apoptosis of BT474 cells after overexpressing MAP2K3. The statistical method is T ‐test. Values were expressed as the mean ± SD from three experiments, and the asterisk indicates the statistical significance compared to the controls ( ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001).

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: AC104024.2 /miR‐30a‐5p Axis Regulates Malignant Progression of Breast Cancer Through a Depression‐Related Gene, MAP2K3

doi: 10.1155/ancp/6608116

Figure Lengend Snippet: MAP2K3 can regulate proliferation, migration, and apoptosis of breast cancer cells. (A) CCK8 assay shows the proliferation ability of BT474 cells after overexpressing MAP2K3. (B) Transwell assay shows the invasion ability of BT474 cells after overexpressing MAP2K3. (C) Apoptosis assay shows the apoptosis of BT474 cells after overexpressing MAP2K3. The statistical method is T ‐test. Values were expressed as the mean ± SD from three experiments, and the asterisk indicates the statistical significance compared to the controls ( ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001).

Article Snippet: After treatment, 100 μL of medium containing 10 μL of CCK‐8 reagent (MCE, HY‐K0301, USA) was added to each well.

Techniques: Migration, CCK-8 Assay, Transwell Assay, Apoptosis Assay

The ceRNA axis AC104024.2 /miR‐30a‐5p/MAP2K3 regulates the malignant progression of breast cancer. (A) The bubble diagrams of GO enrichment analysis in AC104024.2 and miR‐30a‐5p by DAVID database. (B) The CCK8 assays of breast cancer cells were performed when AC104024.2 was knocked down by ASO, and miR‐30a‐5p was overexpressed by mimic for 48 h. (C) The cell apoptosis assays of breast cancer cells were performed when AC104024.2 was knocked down by ASO, and miR‐30a‐5p was overexpressed by mimic for 48 h. The statistical method is T ‐test. Values were expressed as the means ± SD from three experiments, and the asterisk indicates the statistical significance compared to the controls ( ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001).

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: AC104024.2 /miR‐30a‐5p Axis Regulates Malignant Progression of Breast Cancer Through a Depression‐Related Gene, MAP2K3

doi: 10.1155/ancp/6608116

Figure Lengend Snippet: The ceRNA axis AC104024.2 /miR‐30a‐5p/MAP2K3 regulates the malignant progression of breast cancer. (A) The bubble diagrams of GO enrichment analysis in AC104024.2 and miR‐30a‐5p by DAVID database. (B) The CCK8 assays of breast cancer cells were performed when AC104024.2 was knocked down by ASO, and miR‐30a‐5p was overexpressed by mimic for 48 h. (C) The cell apoptosis assays of breast cancer cells were performed when AC104024.2 was knocked down by ASO, and miR‐30a‐5p was overexpressed by mimic for 48 h. The statistical method is T ‐test. Values were expressed as the means ± SD from three experiments, and the asterisk indicates the statistical significance compared to the controls ( ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001).

Article Snippet: After treatment, 100 μL of medium containing 10 μL of CCK‐8 reagent (MCE, HY‐K0301, USA) was added to each well.

Techniques: Analysis